Biotechnological application of biological yeast's invertase: capture and removal of sucrose from fruit juices

Abstract

Alarming numbers gathered by diabetes in the recent years motivated our project with the aim to capture and remove naturally occuring sucrose from fruit juices using the immobilized invertase. The structure and activity of the enzyme was evaluated at different pH values, and solutions with pH 3.0 and 4.8 were chosen to test the sucrose capture and removal. At the end, a removal of approximately 4% of the total sucrose per cycle was achieved.