Palavras-chave
Mutagenicidade
PBTA
Salmonella/microssoma
PBTA
Salmonella/microssoma
Como Citar
AGAPITO, Estevão; UMBUZEIRO, Gisela; VENDEMIATTI, Josiane. Caracterização genotoxicológica de um novo fenil-benzotriazol. Revista dos Trabalhos de Iniciação Científica da UNICAMP, Campinas, SP, n. 27, p. 1–1, 2019. DOI: 10.20396/revpibic2720192760. Disponível em: https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2760. Acesso em: 18 mar. 2026.
Resumo
Os 2-fenil-benzotriazóis (PBTA) são compostos que podem ser formados na reação de redução de corantes dinitrofenil azo, e já foram encontrados em ambientes aquáticos. A mutagenicidade de vários PBTAs já foi descrita na literatura. Neste trabalho avaliamos a mutagenicidade de um novo PBTA, derivado do corante C.I. Disperse Violet 93, encontrado em rios do Estado de São Paulo, utilizando o protocolo miniaturizado (MPA) do ensaio Salmonella/microssoma. Resultados positivos foram obtidos para TA98, com e sem S9, e para TA100, TA97a e YG1041, com S9. A linhagem mais sensivel foi a YG1041 e os dados indicam a importância das enzimas do sistema S9 na atividação do composto.
Referências
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HAGIWARA, Y. et al. Specificity and sensitivity of Salmonella typhimurium YG1041 and YG1042 strains possesing elevated levels of both nitroreductase and acetyltransferase activity. Mutation Research/Environmental Mutagenesis and Related Subjects, v. 291, n. 3, p. 171–180, 1993.
HUNGER, K. Industrial Dyes: Chemistry, Properties, Applications. Frankfurt, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2003.
KUMMROW, F., Umbuzeiro, G.A. 2-Fenilbenzotriazóis (PBTA): Uma nova classe de contaminantes ambientais. Quím. Nova, v. 31, n.2, p. 401–406, 2008.
KANT, R. Textile dyeing industry an environmental hazard. Natural Science, v. 04, n. 01, p. 22-26, 2012.
NUKAYA, H. et al. Isolation and chemical-structural determination of a novel aromatic amine mutagen in water from the Nishitakase River in Kyoto. Chemical Research in Toxicology, v. 10, n. 10, p. 1061-1066, 1997.
OECD. Test No. 471: Bacterial Reverse Mutation Test. [s.l.] OECD Publishing, 1997.
SHIOZAWA, T. et al. Chemical Synthesis of a Novel Aromatic Amine Mutagen Isolated from Water of the Nishitakase River in Kyoto and a Possible Route of Its Formation. Chemical Research in Toxicology. v. 11, p. 375, 1998.
SHIOZAWA, T. et al. Isolation and Identification of a New 2Phenylbenzotriazole-Type Mutagen (PBTA-3) in the Nikko River in Aichi, Japan. Chemical Research in Toxicology. v. 13, n. 7, p. 535–540, 2000.
UMBUZEIRO, G. A. et al., The contribution of azo dyes to the mutagenic activity of the Cristais River. Chemosphere, v.60, n.1, p. 55-64, 2005.
VACCHI, F. I. et al. Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities. Science of The Total Environment, v. 601-602, p. 230-236, 2017.
WATANABE, T. et al.Occurrence and origin of mutagenicity in soil and water environment. Environmental sciences: an international journal of environmental physiology and toxicology, v. 12, n. 5, p. 235-246, 2005.
ZWARG, R. et al. Miniaturization of the microsuspension Salmonella/microsome assay in agar microplates. Environmental and Molecular Mutagenesis, v.59, p. 488-501, 2018.
EPPS, H. H. Basic principles of textile coloration. Color Research & Application, v. 28, n. 3, p. 230-231, 2003.
HAGIWARA, Y. et al. Specificity and sensitivity of Salmonella typhimurium YG1041 and YG1042 strains possesing elevated levels of both nitroreductase and acetyltransferase activity. Mutation Research/Environmental Mutagenesis and Related Subjects, v. 291, n. 3, p. 171–180, 1993.
HUNGER, K. Industrial Dyes: Chemistry, Properties, Applications. Frankfurt, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2003.
KUMMROW, F., Umbuzeiro, G.A. 2-Fenilbenzotriazóis (PBTA): Uma nova classe de contaminantes ambientais. Quím. Nova, v. 31, n.2, p. 401–406, 2008.
KANT, R. Textile dyeing industry an environmental hazard. Natural Science, v. 04, n. 01, p. 22-26, 2012.
NUKAYA, H. et al. Isolation and chemical-structural determination of a novel aromatic amine mutagen in water from the Nishitakase River in Kyoto. Chemical Research in Toxicology, v. 10, n. 10, p. 1061-1066, 1997.
OECD. Test No. 471: Bacterial Reverse Mutation Test. [s.l.] OECD Publishing, 1997.
SHIOZAWA, T. et al. Chemical Synthesis of a Novel Aromatic Amine Mutagen Isolated from Water of the Nishitakase River in Kyoto and a Possible Route of Its Formation. Chemical Research in Toxicology. v. 11, p. 375, 1998.
SHIOZAWA, T. et al. Isolation and Identification of a New 2Phenylbenzotriazole-Type Mutagen (PBTA-3) in the Nikko River in Aichi, Japan. Chemical Research in Toxicology. v. 13, n. 7, p. 535–540, 2000.
UMBUZEIRO, G. A. et al., The contribution of azo dyes to the mutagenic activity of the Cristais River. Chemosphere, v.60, n.1, p. 55-64, 2005.
VACCHI, F. I. et al. Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities. Science of The Total Environment, v. 601-602, p. 230-236, 2017.
WATANABE, T. et al.Occurrence and origin of mutagenicity in soil and water environment. Environmental sciences: an international journal of environmental physiology and toxicology, v. 12, n. 5, p. 235-246, 2005.
ZWARG, R. et al. Miniaturization of the microsuspension Salmonella/microsome assay in agar microplates. Environmental and Molecular Mutagenesis, v.59, p. 488-501, 2018.
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Copyright (c) 2025 Estevão Agapito, Gisela Umbuzeiro, Josiane Vendemiatti