Palavras-chave
S.cerevesiae
N-butanol
Tolerance
N-butanol
Tolerance
Como Citar
INOUE, Heitor; GOLDBECK, Rosana; AZAMBUJA, Suéllen. Selection of wild-type S. cerevisiae strains tolerant to the presence of n-butanol from evolutionary engineering. Revista dos Trabalhos de Iniciação Científica da UNICAMP, Campinas, SP, n. 27, p. 1–1, 2019. DOI: 10.20396/revpibic2720192901. Disponível em: https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2901. Acesso em: 18 mar. 2026.
Resumo
Butanol is a biofuel considered to be the best substitute for gasoline when compared to ethanol. The production of n-butanol from S. cerevisiae is still a challenge due to the low final concentration produced and low tolerance to this alcohol. The objective of this project was to select wild-type strains of S. cerevisiae tolerant to the presence of n-butanol, to carry out evolutionary engineering tests, aiming to increase the tolerance to n-butanol, and to characterize the selected colonies. Two strains were studied, and colonies more tolerant to n-butanol were obtained through evolutionary engineering.
Referências
CHEN, C. T.; LIAO, J. C. FEMS Microbiology Letters, 2016, v. 363, n. 5, p. 1–13.
STEEN, E. J. et al. Microbial Cell Factories, 2008, v. 7, n. 1, p. 36.
VERDUYN, C. et al. Yeast, 1992, v. 8, n. 7, p. 501–517.
STEEN, E. J. et al. Microbial Cell Factories, 2008, v. 7, n. 1, p. 36.
VERDUYN, C. et al. Yeast, 1992, v. 8, n. 7, p. 501–517.
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